Vivacell® 100 is a disposable ultrafiltration unit for the concentration of samples up to 98 ml that can be used either in a centrifuge or for pressure filtration. Vivacell® 100 thus combines the ease of use of centrifugal devices with the flexibility and control provided by pressurized ultrafiltration cells. Vivacell® 100 is easy to assemble, requires no tubing connections or stirring mechanisms and can be adapted to equipment availability or to specific user preferences. For convenience, the units are simply spun in a large capacity centrifuge (carriers accepting 250 ml bottles). For better process control, gas pressure can be combined with gentle orbital shaking. For highest simplicity of use with minimum equipment requirements, the device is pressurized and then left standing on a bench top or in a refrigerator. The concentrator features a vertical membrane that minimizes fouling, while the built-in deadstop of 350 µl will prevent concentration to dryness. Easy to handle High flux PES membrane Vertical twin membrane for ultra-fast concentration High retentate recovery > 90 % Total process flexibility Compatible with swing out rotors Performance Characteristics

Typical performance	Time to concentrate 30x min. at 20°C	Solute recovery %
90 ml Start volume	In centrifuge 2,000 g	As pressure cell 4 bar (58 psi)
	Swing out rotor	No agitation	Orbital shake
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES	22	75	25	96 %
10,000 MWCO PES	16	60	20	96 %
30,000 MWCO PES	16	60	20	94 %
IgG 0.25 mg/ml (160,000 MW)
50,000 MWCO PES	20	70	30	94 %
100,000 MWCO PES	20	85	30	90 %
Latex beads 0.004 % in DMEM 10 % FCS (0.055 µm)
300,000 MWCO PES	35	-	120	99 %
Latex beads 0.004% in DMEM 10% FCS (0.24 µm)
1,000,000 MWCO* PES	4	5	4	99 %

2,000 g in centrifuge, 2 bar (29 psi) pressure